Moringa Oleifera aqueous leaf effect on pancreatic cancer cells.

Apr 27, 2015

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Fewer than 6% patients with adenocarcinoma of the pancreas live up to five years after diagnosis. Chemotherapy is currently the standard treatment, however, these tumors often develop drug resistance over time. Agents for increasing the cytotoxic effects of chemotherapy or reducing the cancer cells’ chemo-resistance to the drugs are required to improve treatment outcome. Nuclear factor kappa B (NF-kB), a pro-inflammatory transcription factor, reportedly plays a significant role in the resistance of pancreatic cancer cells to apoptosis-based chemotherapy. This study investigated the effect of aqueous Moringa Oleifera leaf extract on cultured human pancreatic cancer cells – Panc-1, p34, and COLO 357, and whether it can potentiates the effect of cisplatin chemotherapy on these cells.
METHODS:
The effect of Moringa Oleifera leaf extract alone and in combination with cisplatin on the survival of cultured human pancreatic cancer cells was evaluated by XTT-based colorimetric assay. The distribution of Panc-1 cells in the cell cycle following treatment with Moringa leaf extract was evaluated by flow cytometry, and evaluations of protein levels were via immunoblotting. Data of cell survival following combined treatments were analyzed with Calcusyn software.
RESULTS:
Moringa Oleifera leaf extract inhibited the growth of all pancreatic cell lines tested. This effect was significant in all cells following exposure to ≥0.75 mg/ml of the extract. Exposure of Panc-1 cells to Moringa leaf extract induced an elevation in the sub-G1 cell population of the cell-cycle, and reduced the expression of p65, p-IkBα and IkBα proteins in crude cell extracts. Lastly, Moringa Oleifera leaf extract synergistically enhanced the cytotoxic effect of cisplatin on Panc-1 cells.
CONCLUSION:
Moringa Oleifera leaf extract inhibits the growth of pancreatic cancer cells, the cells NF-κB signaling pathway, and increases the efficacy of chemotherapy in human pancreatic cancer cells.

Source: http://www.ncbi.nlm.nih.gov/pubmed/23957955

Post by Matorwmasen

My name is Clement Matorwmasen. Together with my wife I have co-founded Green Gold Social Enterprise. Since childhood I have always been a social and environmental activist and I was a youth leader in many activist groups. As a child I grew up in difficult circumstances when my parents abandoned me and I grew up at the homes of different foreign missionaries. As a teenager I re-established the contact with my family members and my tribe and I learned I was high up in the royal family lineage. I now have very strong ties to my tribe and I want to make a difference in not only their lives, but that of all Ghanaians. When I reconnected with my family, I was already running several not-for-profit projects trying to improve the living conditions in several poor communities, like organising finances for a community boreholes to improve access to clean drinking water. I was doing this alongside my teaching job as an IT-specialist at a tertiary institution. In 2007 my boss and mentor introduced me to Moringa. I was intrigued and went to learn more about the plant and all its qualities and I saw it as a potential solution to combat malnutrition in our communities. This inspired me to set up my own NGO called Drive Aid Ghana and we started promoting the usage and cultivation of Moringa. I was happy that I could make a difference for some people, but scaling and sustaining our activities were highly problematic. Initially I was able to interest farmers in growing Moringa, but mostly they ended up cutting it down because they were not able to generate income from it. This was because there was no established local market. They also challenged me: ‘if Moringa is so useful and if you can earn so much money with it, then why don’t you do it yourself?’ These experiences made me think and slowly the idea of starting a social enterprise to promote Moringa as a cash crop started to develop. With the full support of my wife I handed over my IT-business to my brother and went full-time into developing our social enterprise Green Gold. We have been donated an abandoned state farm of 3000 ha by one of our communities, which we are currently developing into a Moringa plantation and processing factory. My quest is simply to make a difference in other people’s lives and to make the world a better place one smile at a time, Moringa is my pivot point for sustainable development

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