Evaluation of seed coagulant Moringa oleifera lectin (cMoL) as a bioinsecticidal tool with potential for the control of insects

Abstract

Lectins have demonstrated significant levels of protection against different pests when expressed in transgenic plants. The effects of the coagulant Moringa oleifera lectin (cMoL) on moth flour (Anagasta kuehniella) were tested by incorporating the protein in an artificial diet at levels of 0.5%, 1% and 2% (w/w), respectively. cMoL showed a dose-dependent effect on average larval weight and a series of nutritional disturbances. A significant increase in total development time of 15 days was observed in the group fed with cMoL at 1%, increasing the rate of pupal mortality by 27.6%. The A. kuehniellamidgut proteases were unable to digest cMoL for up to 12 h of incubation. The lectin presented a tight binding to a chitin column, suggesting that the insecticidal activity of cMoL involves carbohydrate–lectin interactions on the surface of the digestive tract, with glycoproteins and others glycolsylated structures in the midgut and resistance to enzymatic digestion.

http://www.sciencedirect.com/science/article/pii/S1359511310003818

Post by Matorwmasen

My name is Clement Matorwmasen. Together with my wife I have co-founded Green Gold Social Enterprise. Since childhood I have always been a social and environmental activist and I was a youth leader in many activist groups. As a child I grew up in difficult circumstances when my parents abandoned me and I grew up at the homes of different foreign missionaries. As a teenager I re-established the contact with my family members and my tribe and I learned I was high up in the royal family lineage. I now have very strong ties to my tribe and I want to make a difference in not only their lives, but that of all Ghanaians. When I reconnected with my family, I was already running several not-for-profit projects trying to improve the living conditions in several poor communities, like organising finances for a community boreholes to improve access to clean drinking water. I was doing this alongside my teaching job as an IT-specialist at a tertiary institution. In 2007 my boss and mentor introduced me to Moringa. I was intrigued and went to learn more about the plant and all its qualities and I saw it as a potential solution to combat malnutrition in our communities. This inspired me to set up my own NGO called Drive Aid Ghana and we started promoting the usage and cultivation of Moringa. I was happy that I could make a difference for some people, but scaling and sustaining our activities were highly problematic. Initially I was able to interest farmers in growing Moringa, but mostly they ended up cutting it down because they were not able to generate income from it. This was because there was no established local market. They also challenged me: ‘if Moringa is so useful and if you can earn so much money with it, then why don’t you do it yourself?’ These experiences made me think and slowly the idea of starting a social enterprise to promote Moringa as a cash crop started to develop. With the full support of my wife I handed over my IT-business to my brother and went full-time into developing our social enterprise Green Gold. We have been donated an abandoned state farm of 3000 ha by one of our communities, which we are currently developing into a Moringa plantation and processing factory. My quest is simply to make a difference in other people’s lives and to make the world a better place one smile at a time, Moringa is my pivot point for sustainable development

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